A better image for your stereo microscope

In compound microscopes, an illumination parallel to the optical axis is the standard. A lot of hardware is implemented to ensure this axial illumination: Center-adjustable Field and Aperture diaphragm, centerable light rings for Phase contrast and Darkfield for transmitted light, center-adjustable objectives and/or centerable rotatable stages in Polarization microscopes. With all these microscopes we are working on 2-dimensional samples.

In stereo microscopy, a 3D image from a 3-dimensional sample is our ambition. Different viewing angles for both eyes create different images on the retina of the left and the right eye. Our brain is going to process both images. This concept is best comprehensible in the Greenough construction of a traditional stereo microscope: two complete separate beam paths from objective to the eyes, mounted in a relative angle of 11°-16°. But even in the modern, more flexible CMO (Central Main Objective) concept a different viewing angle is realized.

Greenough type                       CMO (Galilean) type 

How to avoid extreme highlights from reflecting surfaces

Optimize your illumination system. Extremely applicable in case of image documentation by a digital camera.

Reflected light is polarized light. This physical property is known by everybody who is doing serious photographies with a single lens reflex camera.

Motif: Landscapes, technical buildings, any kind of documentation for newspaper or book.
Solution: A rotatable polarization filter mounted in front of the objective.

Polarization filter of a reflex camera (source)

Rotate the filter until contrast is maximum, reflections are minimum. A new viewing angle requires a new adjustment.

Picture taken with Pol-Filter, interior visible vs Picture taken without Pol-Filter (source)

Selecting the right microscope

AE Series

Category: Advanced Inverted Microscopes for Transmitted light

Target specimen: Cell cultures from microbiology, in flasks or petri dishes, on agar or in fluids; water samples from biology

Target customer: Lab technicians, advanced students

This comparison is an approach for classifying different Inverted microscopes in a defined performance range. Here we are talking about Motic’s AE series, meant for routine work in the microbiology lab of universities and related labs of industrial companies.

The following chart is based on the specifications of the basic outfit of both AE models and its available options. Within this series of publications, our rating always rests upon an individual understanding and validation of the actual numerical values.

AE SERIES - Comparison Chart

Selecting the right microscope

BA Elite Series

Category: Advanced Transmitted Light Microscopes

Target specimen: Transparent samples (sections, smears, emulsions, water samples) from Biology & Medicine; rarely from Industry

Target customer: Lab technicians

This comparison is an approach for classifying different microscope models in a defined performance range. Here we are talking about Motic’s BA Elite series of Advanced Transmitted light microscopes, meant for routine work in university and biomedical lab. The comparison is meant to help you in finding a suitable solution for your lab.

The following chart is based on the specifications of the basic outfit for each model and its upgrade options. Our rating of the single specifications rests upon an individual understanding of the actual numerical values. This is a subjective rating, and we understand that each person might have its own opinion.

BA SERIES - Comparison Chart

Some remarks about FLUORESCENCE

In standard light microscopy FLUORESCENCE is clearly one of the most challenging methods. It requires a clear understanding of the scientific background as well as a proper microscope setup: there are many options to miss a satisfying image result. As the costs of a traditional Mercury illumination setup (still the most flexible approach) are remarkable, a beginner in FLUORESCENCE should take his time to evaluate the adequate microscope & filter hardware.