Artery from another perspective

The preparation of the artery shown in both images is of unknown origin and the staining method is unknown as well.

A fluorescence image of an artery tissue as shown here is not usual. The preparation is more or less evenly green fluorescent colored, because the dye used has attached itself to the structural parts of the preparation. We see a fluorescence color image with details as you might encounter in bright field microscopy with dyes such as e.g. toluidine blue. Such dyes show more or less the same details in the preparation with ordinary bright field microscopy as is shown in the first image.

As mentioned before, visualizing a histological specimen completely by using a fluorescent substance is unusual, because here you apply only one color that shows no specific adhesion, except for proteins, because e.g. fat is uncolored in this preparation. With a normal hematoxylin and eosin staining you have more differentiation, namely in the cell core (DNA, dark blue) and proteins, cytoplasm (pink)

In histology, fluorescence is mainly used after a fluorescent substance has been chemically attached to a specific component present in the tissue.

On the fluorescent image of the preparation the parts of an artery can also be seen. The lumen is filled with green colored red blood cells, then there is a very thin (barely visible) layer of endothelium, the meandering lamina elastica interna (clearly visible) a layer of smooth muscle, the thinner lamina elastica externa (clearly visible), the adventitia (connective  tissue) that turns into adipose (fat) tissue. 

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Selecting the right microscope

AE Series

Category: Advanced Inverted Microscopes for Transmitted light

Target specimen: Cell cultures from microbiology, in flasks or petri dishes, on agar or in fluids; water samples from biology

Target customer: Lab technicians, advanced students

This comparison is an approach for classifying different Inverted microscopes in a defined performance range. Here we are talking about Motic’s AE series, meant for routine work in the microbiology lab of universities and related labs of industrial companies.

The following chart is based on the specifications of the basic outfit of both AE models and its available options. Within this series of publications, our rating always rests upon an individual understanding and validation of the actual numerical values.

AE SERIES - Comparison Chart

Fluorescence a useful phenomenon

Fluorescence microscopy is a variant of light microscopy, which is based on the principle of fluorescence. The property of certain fluorescent substances (fluorochromes) is exploited to absorb light - in this case an FITC-filter was used - and release it again with a different longer wavelength, in this case green. However, there is also the limitation of this type of microscopy, since only samples in which these substances have been introduced or which they naturally contain (auto-fluorescence) can be observed. The fluorescence microscope finds great application above all in biology, biochemistry and biophysics, where it is used for the observation of cell components. because it can also represent structures that are far below the resolution limit. 

If money could grow as easy as baker’s yeast…

To watch the budding, it helps if you focus on one square e.g. the upper left; the restless image on the video is caused by the ‘Brownian movement’, water molecules that collide with the yeast cells.

Take some baker’s yeast and grape sugar and dissolve it in a little water, use a cell culture dish and a Motic inverted microscope. With Motic Images Plus 3.0 ML tuned for time laps, shooting frequency one image per minute, you will get the best results.

Yeast cells use sugars to grow. With sufficient oxygen supply - like in this case - the yeasts completely burn their nutrients into water and carbon dioxide, like all aerobic organisms e.g.:

C6H12O6 + 6H2O + 6O2 ==> 6CO2ꝉ + 12H2O

What is scabies?

Scabies is an infection of the skin caused by a scabies mite. The Latin name for this is 'Sarcoptes scabiei'.

The females of the scabies mite dig small passageways in the top layer of the skin and lay their eggs in it. These eggs come out after three to four days. The animals are grown-up within 18 days. The faeces, the secretions, the eggs and certain other substances cause an allergic reaction to human skin. This reaction causes itching, blisters, bumps, redness and scaling. With a first infection, it takes two to six weeks until these reactions are visible/palpable.

Green curls

Cord moss or Funaria hygrometrica is one of the most common mosses in The Netherlands, especially in severely disturbed and nutrient-rich places such as vacant lots, semi-paved roads etc. A favorite habitat is burning places. A short time after the occurrence of a burn spot, Funaria hygrometrica is almost always present. The spread with spores via the air is extremely effective! In The Netherlands there are few large areas where there is so little disruption that there is no suitable place for Funaria hygrometrica, even if only temporarily.

This is not possible with our epidermis

The scales of a fish are slanted in the skin of the fish and consist of collagen, calcium phosphate and calcium carbonate. They overlap and form a firm layer that protects the fish against injuries, parasites and enemies. In many cases they also serve as reflectors or give colors.

The open structure of a water plant

Hippurus (Hippuris) vulgaris or mare’s-tail is a very common swamp and pond plant. This plant (a helophyte) is counted among the oxygen plants because the underwater leaves also produce a lot of oxygen and resemble water plague (Elodea). But the piece that raises a few decimeters above water looks like a small pine tree. Mare’s-tail can form large masses and continue spreading through means of rhizomes. Mare’s-tail grows preferably on clay soil and especially in brackish water.

A fluorescent flea

Almost daily we have to deal with arthropods such as insects, spiders and their kind around us. Yet we realize less often that these animals can provide a beautiful light show.

Fireflies and other bioluminescent animals produce their luminous shine through a chemical reaction. Other arthropods produce light via fluorescence. At certain wavelengths of light including ultraviolet light, molecules in their exoskeleton absorb this light and radiate it again in a different color with a (longer) wavelength, (there are 'excited electrons' involved, but let's skip the details here)

Protected against harsh conditions

Helm grass is what we encounter in the dunes among others in the Netherlands. The Ammophillia arenaria can withstand the salty water and salty sea air. Ammophillia arenaria can be planted on all types of soil, only on clay soil it is best to make the soil poor with masonry sand. The soil must be calcareous.

A Casuarina cunninghamiana from Australia

The Casuarina cunninghamiana or the River She-Oak is a large, fast-growing, pine-like tree native to the east of New South Wales and Queensland, Australia. The tree, an evergreen, has thin needle-like, flexible green twigs practically without leaves. An image of a cross section thereof is shown here. The epidermis shows a structure which is a little similar to that of pine needles.

The Casuarina cunninghamiana is one of the tallest trees of its kind and can reach a height of 30-35 m. Its straight stem branches are quite low and its bark is greyish brown, rough and cracked so that it often comes loose in strips. The plants can easily be grown from seeds. In some countries it is considered an invasive species because it can surpass indigenous plant communities.

How do you look after four times shedding your skin

An adult female mosquito lays her eggs on the water surface. This can be in a ditch, in pools in the floodplains, in a pond or in the rain barrel. Each species has its own preference. The number of eggs that are laid is very different from species to species and can be up to 300 at a time. After a few days, the larvae crawl out of the egg. These larvae feed on algae, microbes and other substances in the water.

No movement without motor nerve cells

Nerve cells or neurons are cells that you need for example when picking up a pen or in the reaction to contact with a hot or cold object etc. There are three types of nerve cells: sensory, motor and relay nerve cells.

Sensory nerve cells can be found throughout the body. The sensory nerve cells are the cells that collect information, e.g. if something hurts or feels hot or cold etc. The sensory nerve cells send this information to the central nervous system (brain and spinal cord) where it is processed.

Green light from the rocks of the Aladzha Monastery

The remains of the Aladzha Monastery on the Black Sea coast are located just above the port city of Varna, Bulgaria. In the very distant past, this monastery was carved out of a steep rock wall consisting of limestone. The monastery is formed by small caves situated at different levels in the rock wall.

In the photos of some pieces of limestone you can recognize the powdery structure of this soft limestone. 

However, let’s still have a look under the fluorescence microscope then; blue light excitation and FITC filter.  As can you see, green light, this white limestone shows auto fluorescence.

Selecting the right microscope

BA Elite Series

Category: Advanced Transmitted Light Microscopes

Target specimen: Transparent samples (sections, smears, emulsions, water samples) from Biology & Medicine; rarely from Industry

Target customer: Lab technicians

This comparison is an approach for classifying different microscope models in a defined performance range. Here we are talking about Motic’s BA Elite series of Advanced Transmitted light microscopes, meant for routine work in university and biomedical lab. The comparison is meant to help you in finding a suitable solution for your lab.

The following chart is based on the specifications of the basic outfit for each model and its upgrade options. Our rating of the single specifications rests upon an individual understanding of the actual numerical values. This is a subjective rating, and we understand that each person might have its own opinion.

BA SERIES - Comparison Chart

We are not alone

In our body and on our skin live a lot of bacteria. On each human cell there are ten bacterial cells. This total of bacteria on and in our body is also called microbiome. Most of these are found in the large intestine: the intestinal flora. In an adult, the intestinal flora consists of 1014 (which are 100,000,000,000,000 bacteria). 99% thereof is strictly anaerobic.

Seeing the effect of radiation damage

The rock matrix contains quartz, plagioclase feldspar, microcline feldspar and biotite mica. The greenish brown flake of biotite shown in the centre exhibits many dark brown circular features resembling cigarette-type burns. These features are known as pleochroic halos and are a diagnostic feature of biotite. These halos are interesting as they are isotropic compared to the surrounding material which is anisotropic. The halo in the centre of field shows a small brightly coloured crystal of zircon which causes the dark brown effect due to a process called metamictization. This process is the radioactive decay and destruction of the crystal structure surrounding the zircon and the affected area becomes amorphous and hence isotropic.

How a small intestinal roundworm contributed to genetics

Cross-sections at the right place in the uterus of a female Ascaris megalocephala can be used to locate eggs in different stages of the first segmentation mitosis*.

After fixation and coloration of the sections, the chromosomes appear colored. The egg of this parasitic nematode can then serve as a support for studying the chromosomal phenomena that characterize the mitosis.

Surface structures easily revealed

As it is known, revealing the surface structure of minerals and fossils can be done by making an ultra-thin section or an acetate peel. An ultra-thin section is a thin slice of a mineral or fossil mounted on a glass slide and viewed under a microscope. Preparing thin sections produces excellently detailed images, but the techniques are relatively difficult and can require expensive equipment. Making acetate peels is much easier and much cheaper.

Acetate peels are made by polishing a surface, etching it with acid to give it some relief, and then chemically melting a piece of acetate film onto that surface with the

A pest on grapes

Uncinula necator or powdery mildew, a basidiomycete, is a pest on grapes. Its septate hyphae forms a mycelium on the surface of the leaves similar to a spider web. Special hyphal branches, the haustoria, penetrate the host cells to absorb food substances, thereby weakening the grape and reducing the harvest.