Showing posts with label Moticam S12. Show all posts
Showing posts with label Moticam S12. Show all posts

Wednesday, 29 September 2021

Ficus carica petiole and Wacker 3A staining

Vascular bundles can be seen very well in these cross-sectional views of a leaf stem of the fig tree, thanks to the Wacker 3A triple staining. This coloring is done with a mixture containing Astra blue, Acriflavin and Acridine red.


We see the vascular bundles that lie in an outer circle, characteristic of (eu)dicotyledonous plants. In monocotyledonous plants, the bundles are irregularly distributed over the cross section. The Xylem vessels lie on the inward side and the Phloem vessels on the outside of the outer circle. Between Xylem and Phloem we see the intravascular Cambium. Vascular bundles are also present in the inner basic Parenchyma.

Due to the good uniform thickness of the coupe and the fine quality of the Moticam S12 camera, the photos could be taken without stacking.

Monday, 13 September 2021

Kahler's disease

The disease owes its name to the Austrian doctor Otto Kahler, who described the disease as one of the first. Kahler's disease, also called multiple myeloma, is a disease of the bone marrow caused by an uncontrolled proliferation of a certain type of white blood cells: plasma cells (also called plasmocytes). Plasma cells are responsible for the formation of antibodies under normal conditions.

The bone marrow is a spongy substance located in the interior of bones, especially the pelvis, sternum, ribs and vertebrae. Bone marrow plays a role in forming bones, but also in forming the cells of the blood: white blood cells (leucocytes), red blood cells (erythrocytes) and platelets (thrombocytes). The disease develops in one abnormal plasma cell, which divides uncontrollably and whose offspring also divides uncontrollably. Since they are all related, they only synthesize one specific type of antibody (or part of it). Since antibodies are proteins, the antibody produced is referred to as the "M protein" (from Myeloma protein). The name paraprotein is also commonly used for the M protein. When a specific piece (called “light chain”) of a paraprotein is found in the urine (or blood serum), it is called the Bence-Jones protein.

Thursday, 15 July 2021

New Zealand flax

Phormium tenax or New Zaeland flax are evergreen perennials, making a large clump of leathery, strap-shaped leaves coming to a decided point, with tall panicles of small, tubular flowers in summer.

New Zealand flax is an excellent fiber plant, hence the association with real flax (Linum usitatissimum) that is grown in Europe. The fibers of the leaves, up to 2 m long, were used by the Maori to make clothes, bags and shoes, and later the Europeans made rope and sails. All parts of the plant were used: for medicine made from roots, face powder from flower pollen and old blooming stems roped together as rafts.


Wednesday, 12 May 2021

Trypanosoma cruzi

Trypanosoma cruzi is a single-celled parasite, first described in 1909 by the Brazilian physician Carlos Chagas, and the causative agent of Chagas disease. It is a tropical vector disease that occurs in humans, among others, and mainly in South America.

Wednesday, 17 February 2021

Triple Stain Wacker 3A

The Wacker 3A staining method (developed by Robin Wacker) is one of the methods which can be used to distinguish cell differences in plants. The images of the cross section of a Rubus idaeus (raspberry) sprout are showing a nice example:


The method is based on the following solutions: 
  • Astra blue (2% in distilled water)
  • Acriflavin (1% in distilled water) 
  • Acridine red (1% in 50% ethanol).  
*All solutions slightly acidified with 2ml acetic acid per 100ml solution.


Friday, 23 October 2020

Viburnum's annoying ‘hair’

The Viburnum rhytidophyllum or Leatherleaf plant is native to central China and is especially decorative along the roads, even in the shade. The plant is a shrub 3 to 4 m high. The leaves are lance-shaped, 15-20 cm, pendulous, leathery with relief, dark green on top, light green and hairy. ‘Hairy’; that is, occupied with stellate (star-shaped) nonglandular trichomes. Trichomes can have many functions like defence against harmful insects, humidity regulation and even a sensory function. 


Thursday, 13 August 2020

About blood

Blood forms the transport system of the body. It flows continuously to supply oxygen and nutrients to tissue and to remove waste products. It also plays an important role in the defense against pathogens in the body and ensures a constant body temperature. Blood is an important transport medium for hormones. So blood literally means life!

About half of the human blood is plasma. Blood plasma mainly consists of fluid and proteins. The other half of this blood consists of blood cells, namely: red blood cells, white blood cells and platelets. For example, the red blood cells transport oxygen and carbon dioxide through the body. The white blood cells make harmful substances and pathogens recognizable and harmless. Platelets provide crusting to a wound.