A perfect sample for understanding the fine focus procedure: Pollen grains from Cobaea scandens

Cobaea Scandens (Jacob’s ladder family) is a widespread ornamental plant for garden and balcony. Domiciled in Mexico, this climbing plant is cultivated as an annual in the temperate zones.

Beside its aesthetics with bell-shaped violet flowers, there are two more aspects worth to mention:

• This plant is pollinated by bats. 
• Tthe large (~ 160 microns Ø) pollen grains of Cobaea show a complex structure which is worth to discover.

Pollen Grains in Brighfield

Pollen bring light into the darkness

A way of classifying pollen species is by monitoring auto fluorescence images of pollen grains. Several components of the exine (i.e. the outer layer of the pollen wall) show conspicuous auto fluorescence in the pollen grain.