Utilizing the polarization method in light microscopy reveals a specific kind of information about the sample. Applying this contrast method, we less often talk about structure size and shape, resolution power or even a specific staining. In case of transparent samples, polarization contrast in first instance has one subject in mind: the potential BIREFRINGENCE of the sample and its implications on a structural insight.
BIREFRINGENCE is a widespread phenomenon, found in nature as well as in man-made materials. Starch grains, fibers, crystals and minerals, plastic sheets and die cast components, the structural basis is always the same: embedded within an amorphous matrix, structures with an intrinsic geometric pattern cause an impact on the speed of light and its oscillating direction when sent through the sample.
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