While looking through my new stock of prepared slides, I came across this specimen, a section of a petiole of the Castanea sativa. The Castanea sativa or European chestnut is widespread in Europe and Asia, meanwhile also in other temperate zones. The chestnuts are consumed since ancient times by people. The tree can become very old.
It was not necessary to take multiple photos of the object and stack it. The coupe was cut perfectly flat. The section is stained according to the Wacker 3A procedure (Acridine red -Acriflavin - Astra blue).
In the last photo, a few of the many calcium oxalate crystals present in the coupe are clearly made visible, with the aid of crossed polarization filters.
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