Inverted but also ‘upright’

The photos show a cross section of a leaf stalk or petiole of the mahogany tree. A Motic inverted microscope with a fluorescence attachment is used for this. The attachment is equipped with a DAPI long pass filter (excitation at 375 nm).

An inverted microscope is basically intended to view an object through the bottom of a vessel. The material to be studied can for example be present in a Petri dish or cell culture dish. 

If a prepared slide consisting of an object glass with a cover glass, is placed upside down on the stage of the inverted microscope, it will function just like a normal upright microscope. If needed, it can be upgraded with a mechanical stage attachment for a smooth movement of the sample. This is a very practical solution, which is used in this case with a prepared slide of a leaf stalk of the mahogany tree.