Fluorescence microscopy is a variant of light microscopy, which is based on the principle of fluorescence. The property of certain fluorescent substances (fluorochromes) is exploited to absorb light - in this case an FITC-filter was used - and release it again with a different longer wavelength, in this case green. However, there is also the limitation of this type of microscopy, since only samples in which these substances have been introduced or which they naturally contain (auto-fluorescence) can be observed. The fluorescence microscope finds great application above all in biology, biochemistry and biophysics, where it is used for the observation of cell components. because it can also represent structures that are far below the resolution limit.
