Silverberry and the universe

A prepared slide of Silverberry scaly hair is often found in microscopy starters packages for amateurs. The beautiful colors and shapes do well under the microscope.

Basics of Light Microscopy: Incident Light Microscopy for opaque samples (I)

A closer look on Incident Light Microscopy reveals a new and noticeable fact. In a compound microscope for opaque samples, the objective plays two roles. First, like in a Transmitted light setup the objective is part of the imaging process. Quality and performance of the objective are essential elements to get reliable and “close-to-truth” results. 

Secondly, the objective additionally is part of the illumination system. Light from the lamp house passes the illumination axis and is deflected by a 45° orientated semi-transparent mirror. Sent through the objective, the light is reflected by the sample surface and brings back sample information to the eyepieces/camera. For a beginner it is difficult to accept that both illumination and imaging processes do not interfere visually.