A Texas Red and a FITC filter set have been used for taking these fluorescence photographs. The sample, a cross-section of a Convallaria rhizome, is colored with Fast Green and Safranin. It is good to see that with different excitation wavelengths, different structures light up, depending on the filter sets and the fluorochromes used.
It should be noted that structures on a nanoscale, e.g. in living cells, can now be viewed with very advanced fluorescence microscopy techniques. The resolution that is partly achieved with the help of complicated calculation programs and fast computers, is much more than the resolution of conventional light microscopes, which is about 0.2 microns. In 2014 a Nobel Prize was awarded for the development of this super-resolution fluorescence microscopy technique.
Prepared slide by Lieder www.lieder.com
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