In order to obtain maximum performance from your microscope’s optics – good, clear and crisp image – it is very important that the sample is illuminated correctly. For this reason, we would like to share with you some recommendations that will help you get the best image that your microscope can give.
Light and filter.
Turn on the microscope, focus your sample and adjust the light intensity (potentiometer) to an optimal level. Then place the daylight filter (blue filter) on the filter holder. This filter is usually included in the standard package of a halogen or tungsten light microscope. This filter corrects the color temperature, so that the yellow light from the tungsten or halogen bulb becomes white.
Microscopes with LED illumination sources generally do not need this filter, because the color temperature is already high.
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Not using a color balancing filter |
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Using a color balancing filter |
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Field diaphragm. (For microscopes with Koehler illumination)
Place the 10X objective on the